We demonstrated that expression of the two miRNA precursor genes (miR-218-1 and miR-218-2) correlated with expression of the host genes (Slit2 and Slit3, respectively) and that the mature miR-218 was mainly derived from the miR-218-2 precursor, with a concomitant reduction of host Slit3 but not of Slit2 in metastatic GC cells. This evidence concerns the gene SLIT2 and gastric cancer.