Our data demonstrate that the majority of amastigote-infected cells within the core of a granuloma represent KCs that have migrated from neighbouring sinusoids, and by flow cytometry, only this population of KCs expresses detectable Kb-SIINFEKL complexes after infection of mice with OVA-transgenic L. donovani. To determine whether KCs engage in cognate interactions with CD8+ T cell in situ, we used intra-vital 2-photon microscopy to quantify T cell recruitment into and behaviour within individual granulomas. Here, CD8A is linked to infection.