Our results showed that not only the expression of both HSP72 and gp96 in esophageal squamous cell carcinoma was higher than that in tissues adjacent to cancer, but also the expression of both HSP72 and gp96 in esophageal squamous cell carcinomas with metastasis was definitely higher than that of esophageal squamous cell carcinomas without metastasis. Here, HSP90B1 is linked to esophageal squamous cell carcinoma.