At 3, 4, 6, 8 and 12 hours post-infection (hpi), cells were fixed with 4% Paraformaldehyde, permeabilized with Phosphate Buffered Saline (PBS) 0.5% Triton X-100 and incubated for one hour in PBS 0.1% Triton X-100 and 2% Bovine Serum Albumin. The gene discussed is ALB; the disease is infection.