NSCLC cell lines A549 (lung carcinoma), NCI-H520 (squamous cell carcinoma), and NCI-H596 (adenosquamous carcinoma), expressing MFSD2A at very low levels, were stably transfected to over-express MFSD2A (Fig. 3A) and showed a 4-, 16-, and 27-fold reduction in colony number in a colony formation assay, respectively, as compared to their empty vector-transfected counterparts (P < 0.0001) (Fig. 3B). The gene discussed is MFSD2A; the disease is adenosquamous carcinoma.