Quantitation of the results with NIHimage software indicated that there were significant increases in ADH1 and ADH4 proteins in NASH livers: for ADH1, NASH/normal control = 2.8, P<0.001; for ADH4, NASH/normal control = 4.1, P<0.001 (Figure 3B). Here, ADH4 is linked to metabolic dysfunction-associated steatohepatitis.