Some reports investigating the correlation between S100, MMP-7 and CCA nerve invasion have found that expression of MMP-7 in CCA tissue is higher than in latero-carcinoma tissue; moreover, it shows significant correlation with the tumor lymphatic metastasis[32,33], Which indicates that MMP protein might be involved in CCA oncogenesis, and its up-regulation may facilitate CCA metastasis, and which further suggests that activation of MMP-7 is a critical promoting agent of CCA NI; high MMP-7 expression seems to correlate with higher malignancy and less favorable prognosis in CCA sufferers. This evidence concerns the gene MMP7 and cholangiocarcinoma.