In the present study using a different anti-Nkx3.1 antibody raised against mouse Nkx3.1[61], while very intense nuclear staining for Nkx3.1 was seen in virtually all normal luminal cells in the mouse prostate (in all lobes), its expression was dramatically decreased in PIN in all cases in Lo-MYC and Hi-MYC mice examined (Figures 3). The gene discussed is MYC; the disease is prostate intraepithelial neoplasia.