MYC and Familial prostate cancer: Figure 3J shows a clear reduction in Nkx3.1 mRNA in Lo-MYC at 9 weeks of age, a time in which the majority of luminal epithelial cells appear to be PIN cells (see below). Further, in preliminary experiments in human prostate cancer cells that express NKX3.1 (LNCaP cells), knockdown of MYC by siRNA resulted in an increase in NKX3.1 mRNA and protein, showing that MYC can indeed regulate NKX3.1 at the mRNA level (CM Koh, AM De Marzo, manuscript in process).