To examine the role of cytoskeletal remodeling on single-cycle infection with HTLV-1 and HIV-1 vectors in the Jurkat-Raji/CD4 system, cocultures were treated with cytochalasin D (ChD) or jasplakinolide (Jsp), which target actin polymerization/depolymerization, or with nocodazole, which inhibits tubulin polymerization. The gene discussed is CD4; the disease is infection.