As Francisella is trafficking away from the cell membrane during the time course of the infection, the co-localization with TfR1 is lost (Figure 2B and 2E; p = 0.88 for comparison of 15 and 30 minutes timepoints, p = 0.006 for 30 and 45 minute timepoints, and p = 0.61 for 45 and 60 minute timepoints (Student's t-test). Here, TFRC is linked to infection.