TLR4 and ischemia: In conclusion, our study has confirmed that OMT protected the brain from damage caused by MCAO; this effect may be through downregulating the expression of TLR4, TLR2, MyD88, and NF-κB. The elevated expression of TLR4, TLR2, MyD88, and NF-κB is thought to be consistent with ischemia evoked neuron injury and death through inflammatory mechanism.