In order to quantify circulating ANDV-specific T-cells ex vivo and to determine the immunodominant epitopes of ANDV, we first challenged PBMC of 78 Chilean convalescent patients (between 4 months and 13.2 years after hospitalization due to infection) with 310 overlapping peptides (distributed in 13 pools) spanning the entire N- and Gn/Gc precursor proteins [30] in IFN-γ ELISPOT assays. The gene discussed is IFNG; the disease is infection.