To examine the ability of tumour cells to adhere to PMN, 2 × 106 DiI-labelled PMN were directly added to culture wells containing confluent dermal fibroblasts or HMVEC (negative controls), tumour cells, tumour cells with the cocktail of antibodies against adhesion molecules, and HMVEC previously treated with 10 U/mL of human recombinant IL-1β for 5 hours (positive control); all were incubated at 37°C for 30 minutes. The gene discussed is IL1B; the disease is neoplasm.