α-SMA staining for myofibroblasts/HSCs was localised to the fibrotic zone of granulomas and was increased significantly compared with uninfected controls at 7 weeks p.i (1-Way ANOVA, p≤0.001) corresponding with increased collagen staining and the development of fibrosis (Figure 2g–i). Here, ACTA1 is linked to Granuloma.