EGFR and lung carcinoma: As 50 μM of AG825 abolishes phosphorylation of HER2, but not of HER1, in human lung cancer cells (Fernandes et al, 1999), MCF-7/HER2-18 and GCC-BC4 cells were exposed to 50 μM (the estimated IC50 value in a 5-day MTT growth assay, Supplementary Figure S2C; Phatak et al, (2007)) and other concentrations of AG825 for 72 h.