NEFH and esophageal squamous cell carcinoma: Moreover, NEFH expression was reactivated by 5-Aza-dC in all ESCC cell lines, and the reactivation was stronger in TE5, KYSE140, KYSE150, and KYSE520 but decreased or minimally different in TE3 or KYSE410 when both 5-Aza-dC and 300 nM TSA were used (Fig. 1f and data not shown).