To determine if ΔQ-htt can enhance autophagosome synthesis, we transfected SK-N-SH neuroblastoma cells with full-length wild-type (7Q-htt) or ΔQ-htt cDNA expression constructs (diagrams in Figure S5), and monitored the levels of LC3-II 24 h post-transfection by western blotting (Figure 6A). This evidence concerns the gene HTT and neuroblastoma.