To further confirm our hypothesis that loss of CASP8 in breast cancer cells may be due to promoter methylation, we performed MSP, which detected methylation of CpG sites of CASP8 in 5-aza-dc untreated breast cancer cells, MCF-7, MB231, SKBR3, and HCC1937 but not in MCF10 (Figure 3C). The gene discussed is CASP8; the disease is breast cancer.