Up to now, except for interferon-γ and azacytidine, the cytotoxic drugs, 5-Fu and methotrexate, have been shown to upregulate CASP8 and induce cell apoptosis in neuroblastoma, medulloblastoma, Ewing sarcoma, glioblastoma, leukemia, and breast cancer cells [18,19]. This evidence concerns the gene CASP8 and Ewing sarcoma.