EPHA2 and neoplasm: To rule out the possibility that the decreased level of expression in IgG25-treated tumors might be due to altered transcriptional level or to an EphA2 negative cell population arising from the selective pressure of mAb treatment, EphA2 mRNA was quantified in tumor lysates by qPCR, but no variation in the amount of transcript was detected between the different groups (data not shown).