In a second experiment, we confirmed the ability of Treg-conditioned DC to completely abrogate the generation of lethal xenogeneic GVHD; importantly, protection against lethal xenogeneic GVHD conferred by the Treg-conditioned DC was completely abrogated by anti–PD-L1, but not by isotype control antibody (Figure 5B). The gene discussed is CD274; the disease is graft versus host disease.