ANXA5 and pouchitis: Therefore, in order to compare the apoptotic activity in asymptomatic pouches between the highly pouchitis-prone UC patients and the pouchitis-protected patients with FAP we employed immunoblotting, immunoprecipitation assays and histological analysis to determine the expression of pro-apoptotic and anti-apoptotic proteins, and detection of apoptosis by Annexin V fluorescence microscopy in ileal pouch biopsies.