In this paper, we present several procedures which have been performed for the first time in our country, such as: the cultivation and manipulationof U87MG line, the manipulation of athymic – knock–out mice (NUDE Crl: CD–1 Foxn1), the stereotactic inoculation of glioblastomacells and finally the development of glioblastoma xenograft in the brain of inoculated nude mice. The gene discussed is FOXN1; the disease is glioblastoma.