At the protein level, whether in the IBCP group (Fig. 6C, E) or in the WBCP group (Fig. 6D, F), data from several western blot experiments revealed a significant up-regulation of the TLR4 (n = 4, ANOVA1w, P < 0.01, post hoc Dunnett testing) protein in mismatch siRNA- and vehicle-treated bone cancer pain rats relative to that from normal rats. Here, TLR4 is linked to bone neoplasm.