Although we found a modest anti-survival effect of EG3287 on carcinoma A549 and DU145 cells in serum-free medium, we were unable to observe any growth-inhibitory effects of EG3287 alone on carcinoma cells in response to serum stimulation, which suggests that NRP1 plays a less important role in proliferation of these cells. The gene discussed is NRP1; the disease is carcinoma.