EGFR-DN was introduced into Caov-3 cells using adenovirus expressing EGFR-DN [24] or by transient transfection of pcDNA3-EGFR-DN with Amaxa nuocleofector Kit T that yield high transfection efficiency in ovarian cancer cell lines as we described previously [19]. The gene discussed is EGFR; the disease is ovarian carcinoma.