We infected these 14 lines with E. faecalis (as described in the Methods) and then, at five time points post-infection, assayed expression of two antimicrobial peptides that are commonly used as read-outs for the two major immune signaling pathways in Drosophila: DptA for the Imd pathway, and Drs for the Toll pathway. Here, TLR4 is linked to infection.