We first examined whether a point mutation (L30E) in matrix domain of Gag previously reported to abrogate envelope incorporation, infectivity [23] and DRM association [13] in cell lines affect infectivity and modulate envelope association with CD59-enriched compartment in primary CD4+ T cells which are predominantly the natural targets in vivo during entire course of HIV-1 infection. This evidence concerns the gene CD59 and HIV-1 infection.