To verify that RPS3 interacts with NleH1 (Figure 2A), we used an a-RPS3 antibody to immunoprecipitate RPS3 from cell lysates obtained after infection with EPEC UMD207 [eae-/bfp-; [32]] strains expressing FLAG-tagged NleH1, NleH2, or a control FLAG epitope. This evidence concerns the gene RNF112 and infection.