siRNA-mediated MITF knockdown in weakly invasive NZM06 and NZM15 cells caused an average 4-fold increase in migration in transwell assays (Figure 2C; Figure S2), confirming that relative MITF expression was central to the observed difference in invasive potential between Motif 1 and 2 cell lines, and consistent with Motif 1 representing an invasion signature for melanoma cells in vitro. This evidence concerns the gene MITF and melanoma.