While inhibition of MLCK did not lead to dramatic morphological changes (Movie S4), inhibition of either ROCK or NMMII significantly altered cell morphology, with cells exhibiting active membrane ruffles at their leading edges and extending long membrane processes at their trailing edges, consistent with previous observations in other cell systems, including glioma cells (Movie S5) [33], [34]. The gene discussed is MYLK; the disease is glioma.