The results of western blotting were consistent with those of QPCR, CENP-E protein level in HCC tissues (0.267 ± 0.038), as measured by western blot, were diminished by about one-fold as compared with that of the para-cancerous tissues (0.762 ± 0.041)(t = 12.2, P = 0<0.05), and only about half of CENP-E in HepG2 cells (0.257 ± 0.039) extract could be detected as compared in LO2 cell extract (0.759 ± 0.023) (fig. This evidence concerns the gene CENPE and hepatocellular carcinoma.