mRNA for glycolytic enzymes enolase 1 (ENO1), phosphoglycerate mutase 1 (PGAM1), phosphoglycerate kinase 1 (PGK1) and phosphofructokinase (PFKM) were all significantly increased in MADD patients (Figure 5C). This evidence concerns the gene ENO1 and multiple acyl-CoA dehydrogenase deficiency.