Therefore to demonstrate the presence of long-lived antigen-specific PC, MSP-1 specific ELISpot assays were performed, in which infected mice were treated for 4 days with the immunosupressive drug, cyclophosphamide (CY), at different times in the infection (i.e., days 8, 30 and 45, Figure 4), and the number of splenic and bone marrow MSP119-specific ASC determined seven days after the the initiation of the CY treament (Figure 4A). This evidence concerns the gene ATAD1 and infection.