TNFRSF10B and melanoma: However, up-regulation of TRAIL-R2 by 2-DG in the melanoma cell lines, ME4405 that lacks p53 expression [32] and Sk-Mel-28 that harbors mutated p53 [32], suggested that 2-DG-mediated up-regulation of TRAIL-R2 was independent of p53 (Figure 2B).