RENBP and malignant colon neoplasm: Since JCV early transcript region allows expression of all 5 transforming proteins including T-Ag, t-Ag and the three splice variants of large T-Ag (T'165, T'136 and T'135), we performed western immunoblotting with a cocktail of specific JCV antibodies to determine the expression of specific early proteins that are expressed in JCVE transfected colon cancer cell lines.