To determine if the shift from Th2 to Th1 cytokine polarization induced by our combination therapy correlated with changes in the immune cell milieu of the TME, we used immunohistochemistry to identify tumor associated macrophages (TAMs), myeloid derived suppressor cells (MDSCs), T regulatory cells (Tregs), dendritic cells (DCs) and CD8+ T cells in primary tumors. This evidence concerns the gene CD8A and neoplasm.