NKD1 and colorectal carcinoma: Both the colonic epithelial cell line CCD841 and the CRC cell line SW480, the latter with a C>T mutation at APC codon #1338 [20], encode a wild-type Nkd1 (470 aa) of 53.2 kDa that migrates at ∼50 kDa on western blot (Fig. 1D, E).