These results suggest not only that the inhibitory effect of GD3 is not specific for a particular hepatoma cell line, but also that exogenous GD3 antagonizes the c-Src/NF-kB pathway induce by hypoxia, reproducing the effects observed with the stably expression of GD3 synthase in Hep3B-GD3 cells, which minimizes the potential contribution of artifacts during clone selection. The gene discussed is SRC; the disease is hepatocellular carcinoma.