It has also been shown that glycoprotein D (gD) can be replaced by the vesicular stomatitis virus glycoprotein G spike (VSV-G) in the envelope of a pseudotyped recombinant HSV-1 virion and still retain virus entry activity and initiate a productive infection in a gD-complementing VD60 cell line, albeit at a 50% reduced level compared to a wild-type virus containing gD [80]. This evidence concerns the gene PAEP and infection.