In this study we have compared immunogenicity and efficacy of threetypes of DNA vaccines: single antigen Gp63 (Gp63/pcDNA), polytope (Poly/pcDNA)and Polytope fused with hsp70 (Poly/hsp/pcDNA) against visceral leishmaniasis insusceptible BALB/c mice. The gene discussed is LMLN; the disease is visceral leishmaniasis.