Our data shows that these splenocytes were able to lyseLeishmania infected J774A.1 macrophages invitro.The perforin dependent pathway of cytotoxicity could have mediated this cytolyticactivity of splenocytes particularly of CD8+ T cells which have been shownto be functioning in visceral leishmaniasis by Tsagozis et al[46]. Here, CD8A is linked to visceral leishmaniasis.