However, we assessed the expression of eight genes in more detail by employing RT-PCR and real-time PCR, which confirmed the overexpression of BMP4, CFL1, and MRVI1, and the underexpression of ACTA2, GRCC10, TIMP3, TIMP1, and SSTR1 in keratoconus. This evidence concerns the gene TIMP1 and keratoconus.