However, we assessed the expression of eight genes in more detail by employing RT-PCR and real-time PCR, which confirmed the overexpression of BMP4, CFL1, and MRVI1, and the underexpression of ACTA2, GRCC10, TIMP3, TIMP1, and SSTR1 in keratoconus. The gene discussed is IRAG1; the disease is keratoconus.