Data obtained after normalization to GAPDH showed that, in keratoconus, BMP4 increased 1.6 fold (Figure 3A), CFL1 increased 3.3 fold (Figure 3B), MVI1 increased 11 fold (Figure 3C), ACTA2 decreased by nearly 4.5 fold (Figure 3D), GRCC10 decreased by 2.7 fold (Figure 3E), TIMP3 decreased by nearly 14 fold (Figure 3F), TIMP1 decreased by 8.5 fold (Figure 3G), and SSTR1 decreased by 1.8 fold (Figure 3H) relative to a normal cornea. The gene discussed is ACTA2; the disease is keratoconus.