CD248 and brain neoplasm: In order to confirm that TEM1/endosialin induction was common to both primary and secondary brain tumors, we performed quantitative RT-PCR using TEM1/endosialin specific primers on mRNA isolated from frozen clinical specimens of 2 non-neoplastic brain tissues and 5 GBMs, as well as 5 specimens of metastatic lung adenocarcinoma resected from brain.