We next used in situ hybridization and immunohistochemistry to localize expression of TEM1/endosialin mRNA and protein (Fig 1C) using an independent array of 7 non-neoplastic (reactive) brain tissue specimens, 4 pilocytic astrocytomas, 7 metastatic adenocarcinomas (3 lung adenocarcinomas, 1 lung adenosquamous carcinoma, 1 non-small cell lung carcinoma, 1 esophageal adenocarcinoma and 1 colon adenocarcinoma) and 9 GBMs. This evidence concerns the gene CD248 and pilocytic astrocytoma.