While the relatively low uptake of transferrin in the HBE135 cell line did not permit a clear assessment of transferrin accumulation upon monensin treatment, all of the NSCLC cell lines, including the wtEGFR-expressing cell line H1666, showed a marked increase in perinuclear labeled transferrin staining in the presence of monensin, indicating an effective inhibition of cargo exit from the endocytic recycling compartment. The gene discussed is TF; the disease is non-small cell lung carcinoma.