EGFR and non-small cell lung carcinoma: To examine the cell surface versus intracellular (endocytic vesicular) localization of EGFR, we carried out confocal immunofluorescence imaging analyses of a normal bronchial epithelial cell line HBE135 expressing wtEGFR and NSCLC cell lines expressing wtEGFR (H1666) or mutant EGFRs (H1650 and HCC827 expressing EGFR Δ746-750, HCC4006 expressing EGFR Δ747-749/A750P, and H1975 expressing EGFR L858R/T790 M) [4,33].