A set of proteins identified in ovarian cancer cells and found to be upregulated in plasma from tumor bearing mice, TIMP1, LCN2, IGFBP2, PFN1, SPARC, EEF1B2, CLU, and FBLN2, were selected for immunoblot analysis using tumor tissue collected from mouse models, conditioned media from human ovarian cancer cell lines, and primary ovarian tumors from human subjects. The gene discussed is PFN1; the disease is ovarian cancer.