A set of proteins identified in ovarian cancer cells and found to be upregulated in plasma from tumor bearing mice, TIMP1, LCN2, IGFBP2, PFN1, SPARC, EEF1B2, CLU, and FBLN2, were selected for immunoblot analysis using tumor tissue collected from mouse models, conditioned media from human ovarian cancer cell lines, and primary ovarian tumors from human subjects. This evidence concerns the gene EEF1B2 and ovarian cancer.