In order to validate our findings in vivo, we infected Ips-1−/− and littermate Ips-1+/− mice with L. pneumophila (2.5×106 LP01 ΔflaA per mouse, infected intranasally) and assayed type I interferon production in bronchoalveolar lavage fluid 20 hours post infection by bioassay. Here, MAVS is linked to infection.