PRNP and Creutzfeldt Jacob disease: To assess the purity of the 15B3-immunoprecipitated aggregates, purified fractions from uninoculate or RML-inoculated Tg(PG14) mice, Tg(CJD) mice expressing the mouse homologue of the D178N/V129 PrP mutation linked to Creutzfeldt-Jakob disease (CJD) [27], and RML-infected C57BL/6 mice were subjected to SDS-PAGE and analyzed by silver staining or Western blot using anti-PrP antibodies (Fig. 3B and C).