In contrast, 12 weeks of high-fat diet induced statistically significant hyperinsulinemia in NASH rats as compared with control animals (plasma insulin values of 5.22 ± 0.98 versus 2.86 ± 0.21 ng mL−1, resp.; Figure 1(b), P < .05). This evidence concerns the gene INS and metabolic dysfunction-associated steatohepatitis.