One of these presented with the only case of cleft palate.[65] A heterozygous MSX1 nonsense mutation has been identified in a three-generation Dutch family exhibiting various combinations of CLP, CP and selective tooth agenesis.[66] In a follow-up study using 1000 unrelated individuals with CLP, complete sequencing of the gene showed that mutations in MSX1 alone could account for 2% of isolated CL/P.[32]. Here, MSX1 is linked to cleft palate.