The status of the methylation pattern was directly associated with the response to DAC treatment: RCC cell lines with a strongly methylated UCHL1 promoter DNA responded to low concentrations of DAC (1 μM, MZ1851RC), whereas higher DAC doses were required to efficiently demethylate partially methylated promoters (10 μM, MZ2862RC). The gene discussed is UCHL1; the disease is renal cell carcinoma.