UCHL1 and renal cell carcinoma: As determined by RT-PCR and Western blot analysis, 3/17 RCC cell lines express neither UCHL1 mRNA nor protein, 4/17 RCC cell lines exhibit low UCHL1 transcription, but no UCHL1 protein, whereas 9/17 express high levels of UCHL1 mRNA and protein (Table 1; [21]).