Although this led to a significant reduction in the size of ept,H99 clones (paralleling the effect of the DN-DaPKC transgene on ept tumor size), close examination of DN-DaPKC+ept,H99 clones stained with the anti-Rbf1 antibody revealed no obvious difference in Rbf1 levels relative to surrounding normal cells (Fig 3D–D′′). This evidence concerns the gene LGI1 and neoplasm.